The Ground Substance of Abnormal Dentin, Secondary Dentin, and Pulp Calcifications

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Abstract
Carbohydrate-protein complexes of various types of dentin were studied by use of the periodic acid Schiff method in conjunction with fixation by freezing and drying. Experimentally produced abnormal dentin, primary and secondary dentin, odontomes, dentin in dentinogenesis imperfecta, dentin in internal resorption and pulp stones were stained by the above mentioned procedure. Some specimens were treated with various buffers and enzymes prior to staining. The ground substance component of abnormal and interglobular dentin as stained by the periodic acid-Schiff method exhibits a greater stainability than normal mature dentin. Some forms of abnormal dentin contain an appreciable water soluble fraction and are altered by the enzyme collagenase.