A CYTOLOGICAL STUDY OF NOCARDIA CORALLINA AND OTHER ACTINOMYCETES

Abstract
Nocardia corallina, Nocardia blackwelli, and Streptomyces griseus were studied using the Chance crystal-violet nuclear stain, cell wall stains, and phase microscopy. Coccoidal forms of Nocardia possess vesicular appearing nuclei in old cultures. Discrete bodies showing characteristics of nuclei were observed in all stages of growth. Hyphal fragmentation was observed to occur through cross septum formation rather than by cytoplasmic segmentation. Cell plates were formed during cytokinesis in the coccoidal cells. Temps. above 35oC produced greatly enlarged forms which became extremely multicellular.

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