Nucleotide sequence of cDNA encoding human alpha 2-macroglobulin and assignment of the chromosomal locus.
- 1 April 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (8) , 2282-2286
- https://doi.org/10.1073/pnas.82.8.2282
Abstract
Six .alpha.2-macroglobulin (.alpha.2M) cDNA [complementary DNA] clones were isolated from a human liver cDNA library by using synthetic oligonucleotides as hybridization probes. One of these, p.alpha.2M1, carries a 4.6-kilobase-pair insert, which was sequenced. The insert contains the coding sequences for the mature .alpha.2M polypeptide (1451 amino acids) and for a 23-amino acid signal peptide at the NH2 terminus of the precursor pro-.alpha.2M. At the 3'' end of the insert a poly(A) addition signal A-A-T-A-A-A and part of the poly(A) tail of the messenger RNA were found. The protein sequence deduced from the nucleotide sequence agrees with the published .alpha.2M amino acid sequence for all except 3 residues. The .alpha.2M locus was assigned to human chromosome 12 by Southern blot analysis with DNA from a panel of mouse/human somatic cell hybrids, using .alpha.2M cDNA as a hybridization probe.This publication has 49 references indexed in Scilit:
- α2‐MACROGLOBULIN: AN INTRODUCTIONAnnals of the New York Academy of Sciences, 1983
- REACTIVE CENTERS IN ?2-MACROGLOBULINAnnals of the New York Academy of Sciences, 1983
- Simian Sarcoma Virus onc Gene, v- sis , Is Derived from the Gene (or Genes) Encoding a Platelet-Derived Growth FactorScience, 1983
- Platelet-derived growth factor is structurally related to the putative transforming protein p28sis of simian sarcoma virusNature, 1983
- Primary structure of the ‘bait’ region for proteinases in α2‐macroglobulinFEBS Letters, 1981
- Sequence location of the reactive thiol ester in human α2 -macroglobulinFEBS Letters, 1981
- A thiol‐ester in α2‐macroglobulin cleaved during proteinase complex formationFEBS Letters, 1980
- Assignment of the human acid α‐glucosidase gene (αGLU) to chromosome 17 using somatic cell hybridsAnnals of Human Genetics, 1979
- Immunochemical analysis of the N-acetyl hexosaminidases in human-mouse hybrids made using a double selective systemCytogenetic and Genome Research, 1977
- Differential staining of human and mouse chromosomes in interspecific cell hybridsNature, 1974