Cloning of DNA sequences from Methanococcus vannielii capable of autonomous replication in yeast
- 1 December 1985
- journal article
- research article
- Published by Springer Nature in Archiv für Mikrobiologie
- Vol. 143 (3) , 253-255
- https://doi.org/10.1007/bf00411245
Abstract
Total DNA of the archaebacterium Methanococcus vannielii was digested with BamHI or BamHI/HindIII, cloned with plasmid Yip5 and analyzed for sequences capable of autonomous replication (ARSs) in the eukaryote Saccharomyces cerevisiae. Two recombinant plasmids were isolated which contained 3.3 kb and 8 kb fragments of methanogen derived DNA with ARS activity. They exhibited low transformation efficiencies for yeast and promoted slow growth of yeast transformants.Keywords
This publication has 17 references indexed in Scilit:
- The ori sequences of the mitochondrial genome of a wild-type yeast strain: number, location, orientation and structureGene, 1984
- Structural requirements for the function of a yeast chromosomal replicatorCell, 1984
- Characterization of human chromosomal DNA sequences which replicate autonomously inSaccharomyces cerevisiaeNucleic Acids Research, 1984
- Pedigree analysis of plasmid segregation in yeastCell, 1983
- A plasmid in the archaebacterium Methanobacterium thermoautotrophicumMolecular Genetics and Genomics, 1983
- Plasmids from Staphylococcus aureus replicate in yeast Saccharomyces cerevisiaeNature, 1982
- High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 geneProceedings of the National Academy of Sciences, 1979
- High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.Proceedings of the National Academy of Sciences, 1979
- Transformation of yeast.Proceedings of the National Academy of Sciences, 1978
- A complementation analysis of the restriction and modification of DNA in Escherichia coliJournal of Molecular Biology, 1969