Identification by fast atom bombardment mass spectrometry of insulin fragments produced by insulin proteinase
- 31 December 1987
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 249 (1) , 215-222
- https://doi.org/10.1042/bj2490215
Abstract
We describe the isolation by reversed-phase h.p.l.c. of a number of products of the degradation of insulin by insulin proteinase and their direct analysis by fast atom bombardment mass spectrometry (f.a.b.a-m.s.). Various semisynthetically labelled insulins were used, including [[3H2]GlyA1]insulin and [[18O]LysB29]insulin. The results obtained confirm and extend the results obtained by non-mass-spectrometric methods [Davies, Muir, Rose and Offord (1988) Biochem. J. 249, 209-214, and papers cited therein]. Cleavage sites were identified between positions A13-A14, A14-A15, B9-B10 B13-B14, B24-B25 and B25-B26. The advantages and disadvantages of the application of f.a.b.-m.s. to such studies are discussed.This publication has 9 references indexed in Scilit:
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