Identification of radioactive insulin fragments liberated by insulin proteinase during the degradation of semisynthetic [[3H]GlyA1]insulin and [[3H]PheB1]insulin

Publisher Website
Google Scholar
Abstract
(1) We [Muir, Offord and Daves (1986) Bicohem. J. 237, 631-637 and Davies, Muir and Offord (1986) Biochem. J. 240, 609-612] have previously identified a major product in the degradation of insulin by insulin proteinase (the N-terminal fragment produced by a cleavage between residues LeuA13 and TyrA14, SerB9 and HisB10) together with eivdence for a minor cleavage site between HisB10 and LeuB11 or between LeuB11 and VAlb12. (2) We now present evidence for minor sites of cleavage between TyrA14 and GlnA15, GluB15, GluB13 and AlaB14 as well as HisB10 and LeuB11.