2′-Deoxy-2′-fluorouridine-5′-triphosphates: a possible substrate for E. coli RNA polymerase
- 1 January 1979
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 6 (3) , 1041-1048
- https://doi.org/10.1093/nar/6.3.1041
Abstract
DUflTP was tested as substrate in the E. coli RNA polymerase system using poly(dAT) as template. dUflTP could replace UTP when Mn++ was utilized as divalent cation instead of Mg++. The level of transcription with the fluoro analog was then 55% of that with UTP.Keywords
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