A Simple Method for Concentration of Live and Formaldehyde-Inactivated Poliovirus

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Abstract
A simple and efficient method for concentration of poliovirus preparations is described. Bivalent cations, and particularly cobalt chloride, are used as precipitating agents, and the precipitate is resuspended with acid and/or by dialysis. Concentration of both live virus preparations as well as formalinized vaccine (Salk type) may be obtained in this way. The method permits concentration of live poliovirus about one thousand-fold and that of vaccine over one-hundred-fold, with negligible losses of virus or antigen.