CHARACTERIZATION OF AN ESTABLISHED CELL-LINE FROM HUMAN RENAL-CARCINOMA

  • 1 January 1979
    • journal article
    • research article
    • Vol. 39  (11) , 4694-4699
Abstract
A cell line, designated as OUR-10, was established from a renal carcinoma in a Japanese woman. This cell line forms monolayers of polygonal epithelial cells with scattered round or dendritic cells and exhibits multilayering. With EM, differentiated surface structures that resemble the microvilli characteristic of renal carcinomas can be seen even at the 60th transfer. The cells have a hypodiploid karyotype with modal numbers of 39 and 40. No marker chromosomes were seen, but definite nonrandom loss of 3 chromosomes in group D and one in group E were recognized. The doubling time was estimated as approximately 32 h in exponentially growing cultures and the cells formed colonies in soft agar with an average efficiency of 25%. Heterotransplantation into the cheek pouch of immunosuppressed hamsters produced tumors that were histologically similar to the original cancerous tissue. The electrophoretic mobility of .gamma.-glutamyl transpeptidase extracted from the cells coincided with that of a novel isozyme found in human renal carcinoma tissue. The genetic phenotype of the glucose-6-phosphate dehydrogenase was the B phenotype. The antigenic structure of HLA was determined as HLA-A2, 11; B5, 40, which was the same as that of peripheral blood lymphocytes of the woman with renal carcinoma.