T-cell activation by peptide antigen: effect of peptide sequence and method of antigen presentation.

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Abstract
A series of synthetic peptide analogs of a determinant recognized by the ovalbumin-specific, I-Ad-restricted, T-cell hybridoma 3DO-54.8 were synthesized. The resulting peptides were tested for activation of 3DO-54.8 cells by using glutaraldehyde-fixed cells as well as reconstituted membranes as antigen-presenting surfaces. The minimum epitope for activation of this T cell is between 7 and 11 amino acids in length. This region includes 2 important H is residues. The order of preference of the various peptide analogs was the same regardless of the method of antigen presentation. The amount of peptide required for T-cell activation was considerably higher when reconstituted membranes, rather than fixed cells, were used as antigen-presenting surfaces.