Immunochemical Studies with Radioactive Isotope, Similarity and Difference Between Serum Vitellin and Lipovitellin

Publisher Website
Google Scholar
Abstract
When P32-containing serum of laying hens was used as antigen, each antiserum to the serum of laying hens (absorbed and unabsorbed with cock serum, with yolk, and with lipovitellin), precipitated 95% of the radioactivity of P32 in protein P fraction, and 50-65% in lipid P fraction. On the other hand, when p32-containing yolk solution in 10% NaCl was used as antigen, these antisera precipitated only 22-36% of radioactivity in the former fraction, and 55-80% in the latter fraction. The ratio of lipid P to protein P in the precipitates obtained with the sera of laying hens and the above described antisera was 1.1-1.6:1, and, in those obtained with yolk and the antisera was 4.5-8:1, compared with 1.6:1 for the lipovitellin used. The injection of the serum of laying hens, yolk and lipovitellin into rabbits produced antibodies which precipitated a similar lipoprotein. From these results, the authors assume that serum vitellin in the sera of laying hens is different chemically from lipovitellin dissolved in 10% NaCl solution.

This publication has 2 references indexed in Scilit: