The amino acid sequence of the enterotoxin from Clostridium perfringens type A
- 25 March 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 182 (2) , 479-484
- https://doi.org/10.1016/0014-5793(85)80358-6
Abstract
The amino acid sequence of the enterotoxin from Clostridium perfringens type A was determined by analysis of peptides derived from the protein by digestion with trypsin chymotrypsin, thermolysin, pepsin, a lysinespecific protease, S. aureus V8 protease and a proline‐specific protease, and fragments generated by cleavage with cyanogen bromide or by dilute acetic acid in 7 M guanidine HCl. The sequence which is complete except for the definite order of 3 small peptides between residues 88 and 103 consists of 309 amino acids and contains a correction to our preliminary announcement [(1984) FEMS Symp. 24, 329‐330].Keywords
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