THE ROLE OF AMINO GROUPS IN THE BIOLOGICAL AND ANTIGENIC ACTIVITIES OF CLOSTRIDIUM PERFRINGENS TYPE A ENTEROTOXIN
- 1 December 1980
- journal article
- Published by Hindawi Limited in Journal of Food Biochemistry
- Vol. 4 (4) , 201-217
- https://doi.org/10.1111/j.1745-4514.1980.tb00781.x
Abstract
Clostridium perfingens type A enterotoxin has 8 amino groups which are readily reactive with 2, 4, 6-trinitrobenzene sulfonic acid (TNBS) in 20 mM phosphate buffer, pH 6.8, and 10 amino groups which react with TNBS only after denaturation with 6.5 M guanidine · HCl ≤ 25°C. The one sulfhydryl group of enterotoxin is not reactive with 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) in the native molecule (phosphate buffer, pH 6.8), but is reactive in the presence of 0.2% sodium dodecyl sulfate at 25°C. iKeywords
This publication has 23 references indexed in Scilit:
- PURIFICATION AND PHYSICOCHEMICAL PROPERTIES OF AN EXTRA CELLULAR AMYLASE FROM A STRAIN OF BACILLUS AMYLOLIQUEFACIENS ISOLATED FROM DRY ONION POWDERJournal of Food Biochemistry, 1979
- [38] The rapid determination of amino groups with TNBSPublished by Elsevier ,1972
- Quantitation of Clostridium perfringens Type A Enterotoxin by ElectroimmunodiffusionApplied Microbiology, 1972
- Chemical modification of amino groups in staphylococcal enterotoxin BBiochemistry, 1969
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- Study of the Toxin of Clostridium botulinumPublished by Elsevier ,1964
- The Kinetics of α-Chymotrypsin Reactions in the Presence of Added NucleophilesJournal of the American Chemical Society, 1964
- Tissue sulfhydryl groupsArchives of Biochemistry and Biophysics, 1959
- Molecular structural effects produced in proteins by reaction with succinic anhydrideBiochimica et Biophysica Acta, 1958
- [11] Methods for investigating the essential groups for enzyme activityPublished by Elsevier ,1957