Stimulation of DNA synthesis carcinogen-induced hepatocytes in vitro

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Abstract
Relatively simple and rapid analytical procedures involving two sequential HPLC separations were developed for the isolation of methylated purines in the urine of rats administered radiolabeled methylating carcinogens. Following a dose of [3N-methyle-N-nitrosourea (MNU), 7-methyladenine (m7Ade) was detected by chromatography as a urinary methylpurine in addition to the expected 7-methylguanine (m7Gua) and 3-methylademne (m7 (m7 Ade). When methyl-labeled methionine was given to rats concurrently with MNU, urinary m7 Gua was labeled, but no radioactivity was recovered in either of the two methyladenine fractions. The profile of urinary methylated purines following a dose of dimethyl sulfate to the rat was similar. Small amounts of 1-methyladenine (m1 Ade) and 3-methylguanine (m7 Gua were also detected in the urine. The excretion of m3 Ade derived from the methyl group of the carcinogen rather than from the normal precursor for methylation, implies that this adduct, like m3 Ade may serve as an indicator in urine for exposure to methylating carcinogens.

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