Site-specific N-terminal labelling of proteinsin vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry
- 13 November 2007
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- No. 4,p. 480-482
- https://doi.org/10.1039/b716115h
Abstract
N-Myristoyl transferase-mediated modification with azide-bearing substrates is introduced as a highly selective and practical method for in vitro and in vivo N-terminal labelling of a recombinant protein using bioorthogonal ligation chemistry.Keywords
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