Comparison and combination of the starch-gel and filter-paper electrophoretic methods applied to human sera: two-dimensional electrophoresis

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Abstract
The results obtained by the electrophoresis of the proteins of normal and abnormal sera by the starch-gel and filter-paper methods are compared. The considerable increase in information given by the starch-gel method is apparent. The components demonstrated in the gels are correlated with those seen on filter-paper, by the use of a suitable combination of the 2 methods. A two-dimensional electrophoretic system (the 1st dimension on filter-paper, the 2d at right angles in starch-gel) is described in detail. More than 20 serum-protein components can be demonstrated with this system. A nomenclature suitable for describing the components demonstrated by the starch-gel methods is proposed. Several of the components have been identified with specific proteins previously isolated or described by other workers.