Overproduction of the bleomycin‐binding proteins from bleomycin‐producing Streptomyces verticillus and a methicillin‐resistant Staphylococcus aureus in Escherichia coli and their immunological characterisation

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Abstract
The bleomycin‐binding proteins designated BLMA and BLMS, which confer resistance to bleomycin (Bm), from Bm‐producing Streptomyces verticillus ATCC15003 and a methicillin‐resistant Staphylococcus aureus B‐26, respectively, were overexpressed in Escherichia coli. The present study showed that both BLMA and BLMS quench the antibacterial activity of Bm by the binding to the drug. To immuno‐chracterize the Bm‐binding proteins, we constructed a monoclonal antibody against BLMA. The antibody, designated 893‐12, did not cross react to BLMS and another Bm‐binding protein from tallysomycin‐producing Streptoalloteichus hindustanus. Although the ability of Bm to cleavage DNA was eliminated by a binding of BLMA to Bm, as shown by Sugiyama et al. [Gene 151 (1994) 11–15], the Bm‐induced DNA degradation was restored by pre‐incubation of BLMA with the anti‐BLMA monoclonal antibody.