FAILURE OF CANINE ISLET ALLOGRAFTS AND AUTOGRAFTS WITH CYCLOSPORINE

  • 1 January 1985
    • journal article
    • research article
    • Vol. 98  (2) , 324-329
Abstract
Intrasplenic autotransplantation of islet fragments prepared by ductal perfusion constitutes a reproducible system for islet delivery of apancreatic dogs. The animals are normoglycemic from the time of grafting; graft failure is rare. A pancreatic dogs received islet autografts (controls), autografts plus oral cyclosporine and allografts plus oral cyclosporine. Therapeutic blood levels of cyclosporine were documented by radioimmunoassay. Allografts and autografts ceased to function after initial normoglycemia in all animals that received cyclosporine: 4 out of 6 autografts failed. Normoglycemia persisted in the control-autografted animals for the duration of the study. Microscopic sections of the failed grafts demonstrated meager tissue survival but no evidence of rejection by cellular infiltration.