The Isocratic Separation and Indirect Uv Detection of Inorganic Anions and Mono- and Di-Carboxylic Acids On A Low-Capacity Anion Exchange Column

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Abstract
The isocratic separation of inorganic anions, and mono-and di-carboxylic acids on a low-capacity anion exchange column using indirect UV detection was studied. Retention of organic analyte anions on low-capacity anion exchange columns has been attributed to anion exchange, adsorption, or a combination of the two. Previous studies (1) were used to provide the mobile phase conditions for this study. It was found that the mobile phase pH. under the conditions used, had a major affect on the separation and resolution of the different analyte anions studied. Isocratic separations for a complex mixture of inorganic anions, and mono- and di-carboxylic acids at different mobile phase pH's are shown.