PHYSIOLOGICAL AND RADIOISOTOPIC EVIDENCE FOR THE BINDING OF HYDROCORTISONE BY HUMAN LEUCOCYTESIN VITRO1

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Abstract
The ameboid migration of human leucocyles, as measured in capillary tubes, is inhibited by the addition of 10 μg/ml. of hydrocortisone. If the leucocytes are incubated with 10 10 μg/ml, hydrocortisone, washed, and then put into plasma to which no hydrocortisone has been added, the inhibition still occurs. If leucocytes are incubated with 10 10 μg/ml, of hydrocortisone, washed, and then put in plasma to which 10 10 μg/ml, of hydrocortisone is added, no more inhibition occurs than if the hydrocortisone were present only during the incubation or only during the migration period. A period of 5 minutes is sufficient for the incubation with hydrocortisone to cause an inhibition in leucocyte migration. Incubation of leucocytes with the hydrocortisone is effective in a non-protein salt-solution, as well as in plasma. This physiological evidence of the binding of hydrocortisone by leucocytes in vitro is substantiated by the detection of radioactivity in leucocytes after incubation with hydrocortisone- 4-C14.