Methods of paper chromatography of steroids applicable to the study of steroids in mammalian blood and tissues

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Abstract
The use of alumina paper for the chromatography of steroids is described. The elimination of displacement effects due to impurities demands tedious purification of most biological extracts. Partition systems of the aqueous methanol/hydrocarbon type can be run very conveniently on paper if certain special procedures are adopted. Extracts sufficiently pure to be handled by these systems can be simply and rapidly prepared. Special methods for quick concn. of these extracts and their even deposition on filter paper are described. Some applications to pure substances, extracts of adrenal glands, and adrenal venous blood are described. A preliminary examination of the "amorphous fraction" obtained from adrenal extracts by Wintersteiner and Pfiffner (1936) indicates the presence of at least 3 very polar substances with strong u-v. absorption near 254 m[mu], 2 of which appear in adrenal venous blood.