An Antigenic Peptide Produced by Peptide Splicing in the Proteasome
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- 23 April 2004
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 304 (5670) , 587-590
- https://doi.org/10.1126/science.1095522
Abstract
CD8 T lymphocytes recognize peptides of 8 to 10 amino acids presented by class I molecules of the major histocompatibility complex. Here, CD8 T lymphocytes were found to recognize a nonameric peptide on melanoma cells that comprises two noncontiguous segments of melanocytic glycoprotein gp100PMEL17. The production of this peptide involves the excision of four amino acids and splicing of the fragments. This process was reproduced in vitro by incubating a precursor peptide of 13 amino acids with highly purified proteasomes. Splicing appears to occur by transpeptidation involving an acyl-enzyme intermediate. Our results reveal an unanticipated aspect of the proteasome function of producing antigenic peptides.Keywords
This publication has 14 references indexed in Scilit:
- Immune recognition of a human renal cancer antigen through post-translational protein splicingNature, 2004
- Substrate access and processing by the 20S proteasome core particleThe International Journal of Biochemistry & Cell Biology, 2003
- Direct observation of ligand recognition by T cellsNature, 2002
- Producing Nature’s Gene-Chips: The Generation of Peptides for Display by MHC Class I MoleculesAnnual Review of Immunology, 2002
- Protein Splicing and Related Forms of Protein AutoprocessingAnnual Review of Biochemistry, 2000
- DEGRADATION OF CELL PROTEINS AND THE GENERATION OF MHC CLASS I-PRESENTED PEPTIDESAnnual Review of Immunology, 1999
- A peptide recognized by human cytolytic T lymphocytes on HLA-A2 melanomas is encoded by an intron sequence of the N-acetylglucosaminyltransferase V gene.The Journal of Experimental Medicine, 1996
- Utilization of an alternative open reading frame of a normal gene in generating a novel human cancer antigen.The Journal of Experimental Medicine, 1996
- An HLA-A2-restricted tyrosinase antigen on melanoma cells results from posttranslational modification and suggests a novel pathway for processing of membrane proteins.The Journal of Experimental Medicine, 1996
- A mutated intron sequence codes for an antigenic peptide recognized by cytolytic T lymphocytes on a human melanoma.Proceedings of the National Academy of Sciences, 1995