Solid‐phase peptide synthesis without side‐chain hydroxyl protection of threonine
- 1 November 1991
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 38 (5) , 491-493
- https://doi.org/10.1111/j.1399-3011.1991.tb01531.x
Abstract
A peptide containing four threonine residues was synthesised by the solid-phase method using fluorenyl-methoxycarbonylamino acid reactive esters or coupling by preactivation with 1-hydroxybenzotriazole and Castro's reagent. In two separate experiments the synthesis was carried out with or without protection of the side-chain hydroxyl group of threonine as the tert.-butyl ether. Comparison of the crude peptides after deprotection and detachment from the synthesis resin suggests that side-chain protection of threonine is unnecessary under the synthetic conditions employed.Keywords
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