[3H]5-HT binding in post-mortem human cerebral cortex: methodological considerations
- 1 February 1989
- journal article
- research article
- Published by Springer Nature in Journal Of Neural Transmission-Parkinsons Disease and Dementia Section
- Vol. 78 (1) , 53-59
- https://doi.org/10.1007/bf01247113
Abstract
Summary The effects of different membrane preparations and assay conditions on [3H]5-HT binding to post-mortem human cortical tissue was studied. Optimal binding necessitated thorough removal of endogenous 5-HT and this was achieved either by hypotonic lysis or by preincubation of the membranes at 37‡C. Calcium chloride (4 mM) increased specific [3H]5-HT binding. The further addition of ascorbic acid (5.7 mM) or ascorbic acid and clorgyline (10 ΜM) reduced specific [3H]5-HT binding.Keywords
This publication has 19 references indexed in Scilit:
- Brain 5-HT2 receptor binding sites in depressed suicide victimsBrain Research, 1988
- Proposals for the classification and nomenclature of functional receptors for 5-hydroxytryptamineNeuropharmacology, 1986
- Serotonin binding in mouse brains. Some methodological aspectsJournal Of Neural Transmission-Parkinsons Disease and Dementia Section, 1983
- Dual Effects of Ascorbate on Serotonin and Spiperone Binding in Rat Cortical MembranesJournal of Neurochemistry, 1983
- Deamination of 5‐Hydroxytryptamine by Both Forms of Monoamine Oxidase in the Rat BrainJournal of Neurochemistry, 1982
- Decreased [3H]serotonin and [3H]spiperone binding consequent to lipid peroxidation in rat cortical membranesBiochemical and Biophysical Research Communications, 1982
- Possible hazards in serotonin binding assays in human and rat brain: effects of ascorbate, pargyline, and buffer concentrationJournal of Neuroscience Methods, 1982
- The acetylenic monoamine oxidase inhibitors clorgyline, deprenyl, pargyline and J-508: their properties and applicationsJournal of Pharmacy and Pharmacology, 1981
- Discrimination of Multiple [3H]5‐Hydroxytryptamine Binding Sites by the Neuroleptic Spiperone in Rat BrainJournal of Neurochemistry, 1981
- Ascorbate Decreases Ligand Binding to Neurotransmitter ReceptorsJournal of Neurochemistry, 1980