Global Transcriptome Analysis of the Responses of a Fluoroquinolone-Resistant Streptococcus pneumoniae Mutant and Its Parent to Ciprofloxacin
Open Access
- 1 January 2006
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 50 (1) , 269-278
- https://doi.org/10.1128/aac.50.1.269-278.2006
Abstract
Streptococcus pneumoniae M22 is a multidrug-resistant mutant selected after exposure of capsulated wild-type S. pneumoniae NCTC 7465 (strain M4) to ciprofloxacin. DNA microarray analysis comparing the gene expression profiles of strain M22 with those of strain M4 showed that strain M22 constitutively expressed 22 genes at levels higher than those observed in strain M4 under all conditions studied. These included the genes encoding the enzymes involved in branched-chain amino acid biosynthesis and two genes (patA and patB) with sequences suggestive of ABC transporter proteins. Expression of the patA and patB genes was induced by ciprofloxacin in both strains, but in strain M4 it only reached the levels observed in strain M22 after long incubation with high concentrations of ciprofloxacin. The altered expression profile observed with strain M22 suggested that the mutation or mutations acquired during resistance selection bring the cell into a state in which the expression of critical genes is preemptively altered to correct for the potential effects of ciprofloxacin on gene expression in the parent strain.Keywords
This publication has 45 references indexed in Scilit:
- Prediction of Mechanisms of Action of Antibacterial Compounds by Gene Expression ProfilingAntimicrobial Agents and Chemotherapy, 2004
- Msr(A) and related macrolide/streptogramin resistance determinants: incomplete transporters?International Journal of Antimicrobial Agents, 2003
- Accumulation of 10 Fluoroquinolones by Wild-Type or Efflux Mutant Streptococcus pneumoniaeAntimicrobial Agents and Chemotherapy, 2002
- Complete Genome Sequence of a Virulent Isolate of Streptococcus pneumoniaeScience, 2001
- Mosaic Genes and Mosaic Chromosomes: Intra- and Interspecies Genomic Variation of Streptococcus pneumoniaeInfection and Immunity, 2001
- Gene Expression Changes Triggered by Exposure of Haemophilus influenzae to Novobiocin or Ciprofloxacin: Combined Transcription and Translation AnalysisGenome Research, 2001
- Selective Targeting of Topoisomerase IV and DNA Gyrase in Staphylococcus aureus : Different Patterns of Quinolone- Induced Inhibition of DNA SynthesisAntimicrobial Agents and Chemotherapy, 2000
- The effect of reserpine, an inhibitor of multi-drug efflux pumps, on the in-vitro susceptibilities of fluoroquinolone-resistant strains of Streptococcus pneumoniae to norfloxacinJournal of Antimicrobial Chemotherapy, 1997
- In Vivo Selection of Streptococcus pneumoniae, Resistant to Quinolones, Including SparfloxinClinical Microbiology & Infection, 1995
- The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutantsNature, 1989