The analysis of the fatty acids of normal human depot fat by gas-liquid chromatography

Abstract
The sample preparation and gas-liquid-chromato-graphic analysis of the fatty acids in fully hydrogenated and non- hydrogenated human depot fat are described and discussed. Exact scale copies of tracings of representative column runs are shown, together with Tables in which the logarithmic relation of retention volumes of the 40-45 commonly occurring fatty acids to that of a standard, their carbon numbers on both polar and non-polar columns and their percentage concentrations are given. A thorough analysis of the trace fatty acids is presented, including for the first time the series of branched-chain acids from C13 to C24. The difficulties in the gas-liquid-chromatographic analysis of human tissue fat are discussed.