Intron mutations that affect the splicing efficiency of the CYH2 gene of Saccharomyces cerevisiae
- 1 May 1986
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 203 (2) , 300-304
- https://doi.org/10.1007/bf00333970
Abstract
To define the extent of intervening sequences required for efficient splicing of the CYH2 gene in Saccharomyces cerevisiae, we have constructed a series of intron mutations. Artificial intron extensions of more than 300 bp of the natural intron lead to an inhibition of splicing where-as intron deletions lead to a drastic improvement of the splicing efficiency. It is shown that deletion of a 32 bp sequence element within the intron is responsible for this drastic improvement.Keywords
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