Analysis of the structural carbohydrates of herbage
- 1 October 1960
- journal article
- research article
- Published by Wiley in Journal of the Science of Food and Agriculture
- Vol. 11 (10) , 560-566
- https://doi.org/10.1002/jsfa.2740111002
Abstract
A method is proposed for the fractionation of the carbohydrates of plant material by successive extractions with ethanol‐benzene, 95% ethanol and hot water followed by delignification and extraction of the isolated holocellulose with potassium hydroxide solution to yield hemicellulose and α‐cellulose. The polysaccharides in the hot water extract, hemicellulose and α‐cellulose are hydrolysed and the constituent monosaccharides separated by paper chromatography and determined spectrophotometrically.Keywords
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