Human cDNA clones for four species of G alpha s signal transduction protein.
Open Access
- 1 December 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (23) , 8893-8897
- https://doi.org/10.1073/pnas.83.23.8893
Abstract
Lambda gt11 cDNA libraries derived from human brain were screened with oligonucleotide probes for recombinants that code for alpha subunits of G signal transduction proteins. Eleven alpha s clones were detected with both probes and characterized. Four types of alpha s cDNA were cloned that differ in nucleotide sequence in the region that corresponds to amino acid residues 71-88. The clones differ in the codon for alpha s amino acid residue 71 (glutamic acid or aspartic acid), the presence or absence of codons for the next 15 amino acid residues, and the presence or absence of an adjacent serine residue. S1 nuclease protection experiments revealed at least two forms of alpha s mRNA. A mechanism for generating four species of alpha s mRNA by alternative splicing of precursor RNA is proposed.This publication has 27 references indexed in Scilit:
- Identification of Specific Transducin α Subunits in Retinal Rod and Cone PhotoreceptorsScience, 1986
- Primary structure of the α‐subunit of bovine adenylate cyclase‐inhibiting G‐protein deduced from the cDNA sequenceFEBS Letters, 1986
- Primary structure of the α‐subunit of bovine adenylate cyclase‐stimulating G‐protein deduced from the cDNA sequenceFEBS Letters, 1986
- Molecular Cloning of Complementary DNA for the Alpha Subunit of the G Protein that Stimulates Adenylate CyclaseScience, 1985
- Primary structure of the α-subunit of transducin and its relationship to ras proteinsNature, 1985
- Sequence of the Alpha Subunit of Photoreceptor G Protein: Homologies Between Transducin, ras , and Elongation FactorsScience, 1985
- Structure and expression in L-cells of a cloned H4 histone gene of the mouseJournal of Molecular Biology, 1981
- Empirical Predictions of Protein ConformationAnnual Review of Biochemistry, 1978
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Action of single-strand specific nucleases on model DNA heteroduplexes of defined size and sequenceBiochemistry, 1977