Improved Liquid Chromatographic Determination of Procainamide and N-Acetylprocainamide in Serum

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Abstract
A liquid chromatographic method for the determination of procainamide (PA) and N-acetylprocainamide (NAPA) in serum has been developed. This method utilizes isocratic conditions, ambient temperature, and a conventional fixed-wavelength 280-nm detector. Sample pretreatment involves extraction of PA and NAPA, along with p-amino-N-(2-dipropylaminoethyl)-benzamide hydrochloride as internal standard, into an organic phase and reex-traction into an aqueous acidic phase. Using this sample pretreatment, interferences due to commonly used drugs are eliminated. The method accurately measures PA and NAPA to levels as low as I mg/L.