Expression of hepatitis B surface antigen gene in yeast.
- 1 January 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (1) , 1-5
- https://doi.org/10.1073/pnas.80.1.1
Abstract
The DNA sequence coding for hepatitis B virus surface antigen (HBsAg) was placed under control of the repressible acid phosphatase promoter of the yeast Saccharomyces cerevisiae in a plasmid capable of autonomous replication in both yeast and Escherichia coli. Yeast transformed by this plasmid synthesized up to 5 .times. 105 molecules/cell of immunologically active HBsAg polypeptide in phosphate-free medium. The HBsAg polypeptides produced in the yeast cells were assembled into 20-22 nm spherical or oval particles and were immunogenic.This publication has 28 references indexed in Scilit:
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