DC Polarographic and Spectrophotometric Determination of Ampicillin Capsules
- 1 September 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 63 (5) , 1049-1051
- https://doi.org/10.1093/jaoac/63.5.1049
Abstract
Polarographic and spectrophotometric methods are proposed for the determination of ampicillin in capsules. Acidic hydrolysis of ampicillin with 1% HCHO in 0.3N HCl yields a degradation product identified as 2-hydroxy-3-phenyl-6-methylpyrazine. This compound has a well defined UV absorption band at 380 nm and a polarographic wave at –0.55 V vs SCE, which can be used for analytical purposes. Individual capsule assays, composite assays, and recovery studies are described. The average recovery values and standard deviations (SD) for UV and polarographic determinations were 99.20% (SD 0.95) and 100.85% (SD 1.09), respectivelyThis publication has 3 references indexed in Scilit:
- DC Polarography of Cephradine and Its Application to CapsulesJournal of AOAC INTERNATIONAL, 1979
- Polarographic Analysis of CephalexinJournal of Pharmaceutical Sciences, 1978
- Isolation and identification of the fluorescent degradation product of some β-lactam antibioticsJournal of Pharmacy and Pharmacology, 1978