EFFECT OF ANTIFUNGAL AGENT X-5079C ON GROWTH OF HISTOPLASMA CAPSULATUM IN VITRO

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Abstract
The susceptibility of 3 strains of Histoplasma capsulatum to the antifungal polypeptide X-5079C has been determined. The substance was fungistatic rather than fungicidal for this microorganism. The agent lost activity gradually in Sabouraud broth and delayed growth of H. capsulatum occurred in cultures containing the drug. Growth of the yeast form of H. capsulatum at 37[degree]C was inhibited by concentrations of the antifungal agent which have been reported present in the serum of patients undergoing treatment with the drug. The mycelial form of the fungus at 30[degree]C was more resistant to X-5079C than the yeast form. At a concentration of 100 [mu]g/ ml, the substance was not toxic for HeLa cells in culture. The susceptibility of H. capsulatum yeast cells growing in HeLa cell culture was comparable to that in Saburaud broth.