STUDIES ON DEXTRANSUCRASE

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Abstract
Cell-free extracts from strains of L. mesenteroides grown on sucrose produced riboflavinylglucoside from sucrose and riboflavin. The extract utilized only sucrose as a glucosyl donor in the formation of riboflavinylglucoside, while glucose itself was ineffective. The intracellular enzyme which formed riboflavinylglucoside was isolated by (NH4)2 SO4 precipitation from the cell-free extract of the strain NRRL B-1299 grown on sucrose. Maximum activity of the enzyme was observed in the range pH 4.5-5.3 and around 30[degree]. The enzyme was fairly sensitive to heat. Riboflavinyloligosaccharides were formed by the action of the enzyme through the successive transfer of glucosyl group between riboflavin compounds and sucrose.

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