Effect of Recombinant Interferon-γ on Protein Content, Phagocytic, and Cytotoxic Activity of Mouse Peritoneal Macrophages

Publisher Website
Google Scholar
Abstract
We have studied the effect of recombinant murine interferon-γ(rMuIFN-γ) on the protein content, phagocytic activity, and cytotoxicity of mouse peritoneal macrophages (MPM). The aim of this study was to see whether rMuIFN-γ alone could influence these parameters of MPM activity or if an additional stimulus, like elicitation or cultivation with lipopolysaccharide (LPS), was required. The MPM cultures were treated with rMuIFN-γ for 24, 48, or 72 h. Generally, rMuIFN-γ treatment of the cultures increased the protein content of the MPM. MPM were generally cytotoxic and they phagocytized IgG-opsonized Escherichia coli under the experimental conditions of this study. The effects of rMuIFN-γ on phagocytic and cytotoxic activites were complex and highly dependent on the dose and length of treatment. Low or high concentrations may exert opposite effects on the same functions. Addition of a low dose of LPS to the cultures did not generally amplify the rMuIFN-γ-induced alterations of MPM activities. However, the combination of LPS, high doses of rMuIFN-γ, and long incubation time reduced the protein content, suppressed the phagocytic activity, and negatively influenced the viability of the MPM. Thioglycolate-elicited MPM had a higher baseline activity than resident MPM, but the rMuIFN-γ effect on elicited MPM was parallel to the effect on resident MPM.