Involvement of the yeast metacaspase Yca1 in ubp10Δ-programmed cell death
Open Access
- 30 November 2004
- journal article
- Published by Oxford University Press (OUP) in FEMS Yeast Research
- Vol. 5 (2) , 141-147
- https://doi.org/10.1016/j.femsyr.2004.07.005
Abstract
UBP10 encodes a deubiquitinating enzyme of Saccharomyces cerevisiae. Its inactivation results in a complex phenotype characterized by a subpopulation of cells that exhibits the typical cellular markers of apoptosis. Here, we show that additional deletion of YCA1, coding for the yeast metacaspase, suppressed the ubp10 disruptant phenotype. Moreover, YCA1 overexpression, without any external stimulus, had a detrimental effect on growth and viability of ubp10 cells accompanied by an increase of apoptotic cells. This response was completely abrogated by ascorbic acid addition. We also observed that cells lacking UBP10 had an endogenous caspase activity, revealed by incubation in vivo with FITC-labeled VAD-fmk. All these results argue in favour of an involvement of the yeast metacaspase in the active cell death triggered by loss of UBP10 function.Keywords
This publication has 39 references indexed in Scilit:
- Transcriptional Profiling of ubp10 Null Mutant Reveals Altered Subtelomeric Gene Expression and Insurgence of Oxidative Stress ResponsePublished by Elsevier ,2004
- Apoptosis-like yeast cell death in response to DNA damage and replication defectsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2003
- Inactivation of Cdc13p TriggersMEC1-dependent Apoptotic Signals in YeastPublished by Elsevier ,2003
- Apoptosis in yeast: a new model system with applications in cell biology and medicineCurrent Genetics, 2002
- Pheromone Induces Programmed Cell Death in S. cerevisiaeCurrent Biology, 2002
- Involvement of RAD9 -Dependent Damage Checkpoint Control in Arrest of Cell Cycle, Induction of Cell Death, and Chromosome Instability Caused by Defects in Origin Recognition Complex in Saccharomyces cerevisiaeEukaryotic Cell, 2002
- Saccharomyces cerevisiae commits to a programmed cell death process in response to acetic acidMicrobiology, 2001
- Aged mother cells of Saccharomyces cerevisiae show markers of oxidative stress and apoptosisMolecular Microbiology, 2001
- Mechanisms of ApoptosisThe American Journal of Pathology, 2000
- A Yeast Mutant Showing Diagnostic Markers of Early and Late ApoptosisThe Journal of cell biology, 1997