Monolayer cultures of animal cells with the gyrogen equipped with tubes

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Abstract
The gyrogen with tubes has been the object of repeated trials carried out over a period of some years. The aim of these trials was essentially to multiply BHK cells in monolayer and to reproduce the FMD virus in one operation. At the laboratory stage, the gyrogen represented three roller flasks; at the pilot stage, 100 roller flasks; and at the industrial stage, 500 flasks. Culturing cells on a glass support, is not in itself new, but the difficulty appeared when replicating the virus in order to obtain the high parameters necessary for vaccine production. Everything was accomplished so that finally the cell culture and viral multiplication could be carried out in situ in the same apparatus.