An in vitro Assay of Sucrose Uptake by Developing Bean Cotyledons
- 1 January 1981
- journal article
- research article
- Published by CSIRO Publishing in Functional Plant Biology
- Vol. 8 (2) , 221-235
- https://doi.org/10.1071/pp9810221
Abstract
An in vitro procedure, based on net rates of sucrose uptake from [14C]sucrose solutions, was developed to determine the in vivo sucrose uptake rates of French bean (Phaseolus vulgaris L.) cotyledons. Net rates of in vitro sucrose uptake exhibited saturation kinetics and temperature dependency with Q10 values in the range 1.5-2.0. Optimal rates of net sucrose uptake were obtained in unbuffered media containing sucrose alone. Under sink-limited growth conditions, where in vivo free-space sucrose concentrations saturated (i.e. 90-145 mM) the sucrose uptake process, the in vitro procedure could account for in vivo gains of sucrose by the cotyledons.Keywords
This publication has 6 references indexed in Scilit:
- Changes in Weight, Nitrogen Accumulation, Respiration and Photosynthesis During Growth and Development of Seeds and Pods of Phaseolus vulgarisAmerican Journal of Botany, 1978
- A proton-cotransport system in a higher plant: Sucrose transport in Ricinus communisPlant Science Letters, 1977
- Phloem Loading of SucrosePlant Physiology, 1977
- In vitro Culture of Immature Cotyledons of Soya Bean (Glycine max L. Merr.)Annals of Botany, 1977
- Sugar Uptake and Translocation in the Castor Bean Seedling I. Characteristics of Transfer in Intact and Excised SeedlingsPlant Physiology, 1967
- Organic Growth Factor Requirements of Tobacco Tissue CulturesPhysiologia Plantarum, 1965