Enhanced Post-Thaw Viability of Cryopreserved Cells by Oxygenated Perfluorocarbon orPluronic® F-68

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Abstract
Viability and growth following cryopreservation have been examined following (i) culture of Oryza sativa cv. Taipei 309 (Japonica rice) in medium overlaying oxygenated perfluorocarbon (PFC) liquid (Flutec® PP6), and (ii) culture of two cvs. of Japonica rice (Taipei 309, Tarom), and Lolium multiflorum in media with Pluronic® F-68 (0.01–0.2% w/v). The mean viability of cv. Taipei 309 rice cells following 4 days in medium overlaying oxygenated Flutec® (0.45 ± 0.07; n = 20) was significantly (P < 0.05) greater than control (0.35 ± 0.08). Cell viability of cv. Tarom was increased by 400% (P < 0.001) with 0.1% (w/v) Pluronic® F-68 in the recovery medium. Mean cell viability was also elevated 3-fold (P < 0.001) and 2-fold (P < 0.05) with 0.2% (w/v) and 0.01% (w/v) Pluronic, respectively. A 2-fold increase (P < 0.001) in viability occurred for Taipei 309 cells exposed to 0.01% (w/v) Pluronic® F-68; that of Lolium cells was increased by 31% (P < 0.01) under similar conditions. Pluronic® F-68 (0.01% w/v) also stimulated biomass, by 29–32%, in both cvs. Taipei 309 and Lolium at 30 days post-thawing.