Site-specific mutagenesis of AIDS virus reverse transcriptase
- 25 June 1987
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 327 (6124) , 716-717
- https://doi.org/10.1038/327716a0
Abstract
Human immunodeficiency virus (HIV) is the causative agent of AIDS (acquired immune deficiency syndrome)1–3 a disease which iposes a serious challenge to modern medicine. If we are to conquer this disease we will need a protective vaccine or effective drugs able to block the life cycle of the virus. An early stage in the invasion of the host cell is the conversion of the RN A genome of the virus to a double-stranded DNA intermediate which subsequently becomes integrated into the host cell chromosome4. The enzyme reverse transcriptase is crucial in this process and is thus an obvious chemotherapeutic target. In this study we have used site-directed mutagenesis of this enzyme expressed in Escherichia coli to reveal several important functional regions of the protein including putative components of the triphosphate binding site and pyrophosphate exchange sites.Keywords
This publication has 12 references indexed in Scilit:
- Computer analysis of retroviral pol genes: assignment of enzymatic functions to specific sequences and homologies with nonviral enzymes.Proceedings of the National Academy of Sciences, 1986
- Selection and characterisation of acyclovir-resistant herpes simplex virus type 1 mutants inducing altered DNA polymerase activitiesVirology, 1985
- Isolation of Lymphocytopathic Retroviruses from San Francisco Patients with AIDSScience, 1984
- Sequence similarity among retroviruses—erratum*Nature, 1984
- Detection, Isolation, and Continuous Production of Cytopathic Retroviruses (HTLV-III) from Patients with AIDS and Pre-AIDSScience, 1984
- Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial virusesNucleic Acids Research, 1984
- Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virusNature, 1983
- Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS)Science, 1983
- Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNANucleic Acids Research, 1982
- RNA-Directed DNA Synthesis and RNA Tumor VirusesPublished by Elsevier ,1972