CbbR, a LysR-type transcriptional regulator fromHydrogenophilus thermoluteolus, binds twocbbpromoter regions
Open Access
- 1 May 2001
- journal article
- research article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 198 (2) , 151-157
- https://doi.org/10.1111/j.1574-6968.2001.tb10635.x
Abstract
The cbbR encoding the LysR-type transcriptional regulator is located downstream of cbbLSQOYA and this gene is located upstream of cbbFPT in divergent transcription. The two promoter regions with LysR-binding sites are located in the cbbL upstream region and in the cbbR –cbbF intergenic region. Electrophoretic mobility shift assays using a cell extract of Escherichia coli harboring a plasmid containing cbbR and the DNA fragments of promoter regions indicated that CbbR binds in both regions. NADPH caused differences in the complex of CbbR and DNA.Keywords
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