Electrophoretic and Immunochemical Study of the Lipopolysaccharides Produced by Chemostat-grown Escherichia coli 0157
- 1 September 1987
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 133 (9) , 2679-2687
- https://doi.org/10.1099/00221287-133-9-2679
Abstract
Two chemically different O-polysaccharides, a low molecular mass form of LPS and core LPS produced by chemostat-grown E. coli O157, were analysed by SDS-PAGE, silver staining and immunoblotting. The reactivities of the different O-polysaccharides with antisera prepared against E. coli O157 grown in batch culture, Salmonella O30 or Brucella abortus were very similar, showing that the O-polysaccharides share at least some antigenic determinants. The reactions of the low molecular mass LPS with the antisera indicated it was semi-rough LPS having one repeat unit of the O-polysaccharide attached to core LPS.This publication has 14 references indexed in Scilit:
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