Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels

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Abstract
The morphological heterogeneity of lipopolysaccharides (LPS) among salmonella mutants with different LPS chemotypes was analyzed in Ag-stained polyacrylamide gels. The biochemical differences in the LPS chemotypes were reflected in the unique profiles of the purified LPS. The LPS profiles in the whole-cell lysates were also unique for each chemotype. (Whole-cell lysates were assessed by a method which preferentially Ag stains LPS and by a proteinase K digest of whole-cell lysates. The Ag-stained LPS profiles of proteinase K-digested lysates were similar to the homologous purified LPS and could be used to preliminarily characterize the LPS chemotype before purification. Biochemical variation in LPS composition can be detected in Ag-stained polyacrylamide gels.