Immunoaffinity‐purified opiate receptor specifically binds the δ‐class opiate receptor ligand, cis‐(+)‐3‐methylfentanylisothiocyanate, SUPERFIT

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Abstract
Using an antibody generated against the opiate receptor on NG108‐15 cells, we recently purified the putative receptor from this hybrid cell line. We herein report that the purified receptor complex specifically binds tritiated cis‐(+)‐3‐methylfentanylisothiocyanate (SUPERFIT), with the predominant binding associated with a 58 kDa polypeptide chain. Consistent with these findings is the in situ labeling of a 58 kDa protein with [3H]SUPERFIT on NG108‐15 cells.