Isolation and characterization of puromycin-resistant clones from cultured mosquito cells
- 1 July 1982
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 8 (4) , 521-532
- https://doi.org/10.1007/bf01538712
Abstract
We have isolated from an established Aedes albopictus(mosquito) cell line clones which are resistant to the antibiotic puromycin. On the basis of growth and plating efficiency, clones Pur-8026 and Pur-8612 were five- and seven-fold more resistant, respectively, to puromycin than wild-type cells. In vitro protein synthesis was resistant to puromycin only in extracts prepared from Pur-8612 cells. Measurements of puromycin transport, crossresistance to colchicine, and sensitivity to Tween-80 indicated that resistance in Pur-8026 cells was due to membrane alteration(s) affecting permeability to puromycin. This is the first description of puromycin resistance in insect cells and also the first report of puromycin resistance in an animal cell variant associated with an alteration at the level of protein synthesis.Keywords
This publication has 30 references indexed in Scilit:
- Puromycin resistance in Chinese hamster cells: Genetic and biochemical studies of partially resistant, unstable clonesMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1980
- Togaviruses in Cultured Arthropod CellsPublished by Elsevier ,1980
- Emetine resistance in Chinese hamster cells is linked genetically with an altered 40S ribosomal subunit protein, S20.Proceedings of the National Academy of Sciences, 1979
- DRUG RESISTANCE AND MEMBRANE ALTERATION IN MUTANTS OF MAMMALIAN CELLSCanadian Journal of Genetics and Cytology, 1975
- Photoincorporation of puromycin and N-(ethyl-2-diazomalonyl)puromycin into Escherichia coli ribosomes.Proceedings of the National Academy of Sciences, 1975
- Studies on the Binding of N-Bromoacetylpuromycin and N-Bromoacetylaminonucleoside to Rat Liver RibosomesHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1975
- Studies on proteins of animal ribosomes. Affinity labeling of rat liver ribosomes by N‐bromoacetylpuromycinFEBS Letters, 1974
- Irreversible binding of N‐iodoacetylpuromycin to E. coli ribosomesFEBS Letters, 1973
- ESTIMATION OF MUTATION RATES WITH MAMMALIAN CELLS IN CULTUREProceedings of the National Academy of Sciences, 1959
- ISOLATION AND STUDY OF MUTANTS FROM MAMMALIAN CELLS IN CULTUREProceedings of the National Academy of Sciences, 1959