A Spectrophotometric Method for the Simultaneous Determination of Myoglobin and Hemoglobin in Extracts of Human Muscle.

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Abstract
The differential detn of human Hb and Mgb in a mixture of both pigments can be made with good accuracy, using the Beckman spectrophotometer. Accurate spectrophotometric constants for the CO compounds of Hb and Mgb between 600 and 520 m[mu] and for the reduced pyridine hemochromogens formed from these substances are the basis of the method. A method for obtaining suitable extracts from human heart and skeletal muscle-tissue is described.

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