Emerging technologies for gene manipulation in Drosophila melanogaster
- 1 March 2005
- journal article
- review article
- Published by Springer Nature in Nature Reviews Genetics
- Vol. 6 (3) , 167-178
- https://doi.org/10.1038/nrg1553
Abstract
The popularity of Drosophila melanogaster as a model for understanding eukaryotic biology over the past 100 years has been accompanied by the development of numerous tools for manipulating the fruitfly genome. Here we review some recent technologies that will allow Drosophila melanogaster to be manipulated more easily than any other multicellular organism. These developments include the ability to create molecularly designed deletions, improved genetic mapping technologies, strategies for creating targeted mutations, new transgenic approaches and the means to clone and modify large fragments of DNA.Keywords
This publication has 99 references indexed in Scilit:
- A piggyBac Transposon Gene Trap for the Analysis of Gene Expression and Function in DrosophilaGenetics, 2004
- TILLING. Traditional Mutagenesis Meets Functional GenomicsPlant Physiology, 2004
- TILLING — a high-throughput harvest for functional genomicsNature Reviews Genetics, 2004
- Sex-peptide is the molecular basis of the sperm effect in Drosophila melanogasterProceedings of the National Academy of Sciences, 2003
- Chimeric Nucleases Stimulate Gene Targeting in Human CellsScience, 2003
- Genetic Engineering Using Homologous RecombinationAnnual Review of Genetics, 2002
- Site-specific genomic integration produces therapeutic Factor IX levels in miceNature Biotechnology, 2002
- A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in DrosophilaProceedings of the National Academy of Sciences, 2001
- The Genome Sequence of Drosophila melanogasterScience, 2000
- Ten Years of Enhancer Detection: Lessons from the FlyPlant Cell, 1999