Glycoproteins Containing Peanu t‐Agglutinin Receptors from Human‐Peripheral‐ Blood T‐Lymphocyte Plasma Membranes

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Abstract
A glycoprotein fraction possessing peanut agglutinin receptors has been isolated from detergent extracts of neuraminidase‐treated human peripheral blood T‐lymphocyte plasma membranes with affinity matrices comprising the peanut agglutinin co‐valently immobilised on Sepharose 4B.This fraction could be specifically eluted from affinity columns using buffer solutions supplemented with 0.2 M d‐galactose and was shown, by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (0.1 %), to contain four major glycoprotein components with apparent molecular weights of 200000, 190000, 110000 and 95000.It is suggested, from the observed reactivity of these glycoproteins with various lectins in double‐diffusion experiments, that they possess both O‐glycosidically and N‐glycosidically linked carbohydrates.