Proteinkristalle und Tubuli-Bündel in Hefezellen III. Identifizierung der Kristalle als Alkoholdehydrogenase (ADH)

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Abstract
Inducibility of alcohol dehydrogenase (ADH) crystals in yeast cells depends on the growth phase. They accumulate during exponential growth, a process that is parallelled by accumulation of a special protein (H protein). The following pieces of evidence show that crystals, H protein, and ADH are identical: (1) The three proteins have the same amino acid composition and subunit molecular weight; (2) active H protein and ADH exhibit the same electrophoretic mobility, indicating a common molecular weight of about 158 000 D; (3) the native H protein and a crystal fraction stabilized by Zn++ and dialyzed against EDTA possess ADH activity.