HORSERADISH-PEROXIDASE - KINETIC-STUDIES AND OPTIMIZATION OF THE PEROXIDASE-ACTIVITY DETERMINATION WITH THE SUBSTRATES H2O2 AND 3,3',5,5'-TETRAMETHYLBENZIDINE

  • 1 January 1985
    • journal article
    • research article
    • Vol. 23  (8) , 453-460
Abstract
Horseradish peroxidase is often used as a labeling and indicator enzyme in enzyme immunoassays. To optimize the peroxidase activity determination, the kinetics of the catalytic reaction were investigated in relation to the concentration of H2O2 and 3,3'',5,5''-tetramethylbenzidine, at different pH values, reaction temperatures and incubation times. On the basis of the results, a test procedure is presented, which enables the quantitative determination of peroxidase in the range 0-200 ng/1. In addition, methods are described for the determination of 3,3'',5,5''-tetramethylbenzidine and H2O2.